LOCUS       sequence-97363-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A
            nickase and U6>sgRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Gokcezade J, Sienski G, Duchek P
  TITLE     Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome 
            Editing in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126.
  PUBMED    25236734
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126."
COMMENT     Sequence Label: pDCC5
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(145..165)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc
       61 acagaagtaa ggttccttca caaagatcct gctagcttac gtcaccactt tgtacaagaa
      121 agctgggtcc tattagctgg cctccacctt tctcttcttc ttggggctgt cgcctcccag
      181 ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc
      241 gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt
      301 gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat tcttggcctg
      361 ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt ccagattagc
      421 gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct cgtccaggta
      481 gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg agcccttcag
      541 cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca gggccagttc
      601 gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt tttccagctc
      661 gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt tgtagccctt
      721 ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga tggtgatccc
      781 cagcagctc
//
