LOCUS       sequence-97373-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A
            nickase and U6>sgRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Gokcezade J, Sienski G, Duchek P
  TITLE     Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome 
            Editing in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126.
  PUBMED    25236734
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126."
COMMENT     Sequence Label: pDCC5
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     202..219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        314..713
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      241 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact cgtgaaaggt
      301 aggcggatca gcggttcgac ttgcagcctg aaatacggca cgagtaggaa aagccgagtc
      361 aaatgccgaa tgcagagtct cattacagca caatcaactc aagaaaaact cgacactttt
      421 ttaccatttg cacttaaatc cttttttatt cgttatgtat actttttttg gtccctaacc
      481 aaaacaaaac caaactctct tagtcgtgcc tctatattta aaactatcaa tttattatag
      541 tcaataaatc gaactgtgtt ttcaacaaac gaacaatagg acactttgat tctaaaggaa
      601 attttgaaaa tcttaagcag agggttctta agaccatttg ccaattctta taattctcaa
      661 ctgctctttc ctgatgttga tcatttatat aggtatgttt tcctcaatac ttcgggtctt
      721 cgagaagacc tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      781 cttg
//
