LOCUS       sequence-97374-e         500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human RPL30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-RPL30i1
FEATURES             Location/Qualifiers
     source          1..500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..140)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     144..164
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataacctg
       61 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa cttcgtatag
      121 catacattat acgaagttat aaacagcaca aaaggaaact caccctaact gtaaagtaat
      181 tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgttaacgc gcggtgaccc
      241 tcgaggcagt ggcaataata ttgaactgtt ctagagacag ttcaatatta ttgccactgc
      301 ttttttggat ccattaggcg gccgcgtgga taaccgtatt accgccatgc attagttatt
      361 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      421 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      481 taatgacgta tgttcccata
//
