LOCUS       sequence-97379-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A
            nickase and U6>sgRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Gokcezade J, Sienski G, Duchek P
  TITLE     Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome 
            Editing in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126.
  PUBMED    25236734
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126."
COMMENT     Sequence Label: pDCC5
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(308..328)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(332..358)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(364..388)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtctgtggaa gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat
       61 agcagatccg gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg
      121 cctcggctgt ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt
      181 gccggtcggt gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga
      241 tcacggccca gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtcgg
      301 acgcttcgac cttgcgcttt ttcttcggcg aagcgtaatc tggaacatcg tatgggtaca
      361 ttgagcctgc ttttttgtac aaacttgtat accggtagag ctccgaattc actgcagtgg
      421 taccgcctag gtgtatacct ccggaagcgc gcactcgaga ttcgaacaag cttatcgagc
      481 ttggctgcag attgtttagc ttgttcagct gcgcttgttt atttgcttag ctttcgctta
      541 gcgacgtgtt cactttgctt gtttgaattg aattgtcgct ccgtagacga agcgcctcta
      601 tttatactcc ggcgctcttt tcgcgaacat tcgaggcgcg ctctctcgaa ccaacgagag
      661 cagtatgccg tttactgtgt gacagagtga gagagcatta gtgcagagag ggagagaccc
      721 aaaaagaaaa gagagaataa cgaataacgg ccagagaaat ttctcgagtt ttctttctgc
      781 caaacaaatg acctaccaca ataaccagtt tgttttggga ttctagagcc atttgtctgc
      841 agaattggcg cacgcgctaa aaacggacta gcctta
//
