LOCUS       sequence-97380-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-Ti2
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(102..135)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     139..159
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata atctgttctt
       61 tttaatacta gctacatttt acatgatagg cttggatttc tataacttcg tatagcatac
      121 attatacgaa gttataaaca gcacaaaagg aaactcaccc taactgtaaa gtaattgtgt
      181 gttttgagac tataaatatc ccttggagaa aagccttgtt tgcagtccta ctttagtgag
      241 ataattctag agattatctc actaaagtag gactgctttt ttctcgagta ctaggatcca
      301 ttaggcggcc gcgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
      361 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      421 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      481 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      541 aactgcccac ttggcagtac atcaagtgta tcatatgcca ag
//
