LOCUS       sequence-97381-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A
            nickase and U6>sgRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Gokcezade J, Sienski G, Duchek P
  TITLE     Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome 
            Editing in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126.
  PUBMED    25236734
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126."
COMMENT     Sequence Label: pDCC5
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttcgctcag gccgcctctc tcggccttgg tcagattgtc gaactttctc tgggtaatca
       61 gcttggcgtt cagcagctgc cgccagtagt tcttcatctt cttcacgacc tcttcggagg
      121 gcacgttgtc gctcttgccc cggttcttgt cgcttctggt cagcaccttg ttgtcgatgg
      181 agtcgtcctt cagaaagctc tgaggcacga tatggtccac atcgtagtcg gacagccggt
      241 tgatgtccag ttcctggtcc acgtacatat cccgcccatt ctgcaggtag tacaggtaca
      301 gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc tttcaggatc tggctgccca
      361 gctctttgat gccctcttcg atccgcttca ttctctcgcg gctgttcttc tgtcccttct
      421 gggtggtctg gttctctctg gccatttcga tcacgatgtt ctcgggcttg tgccggccca
      481 tcactttcac gagctcgtcc accaccttca ctgtctgcag gatgcccttc ttaatggcgg
      541 ggctgccggc cagattggca atgtgctcgt gcaggctatc gccctggccg gacacctggg
      601 ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc atgaagtttc
      661 tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt cttgccggac tgcttgtccc
      721 ggatgccgtt gatcagcttc cggctcagcc tgccccagcc ggtgtatctc cgccgcttca
      781 gctgcttcat cactttgtcg tcgaacaggt gggcataggt tttcagccgt tcctcgatca
      841 tctctctgtc ctc
//
