LOCUS       sequence-97386-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and 
            U6>sgRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Gokcezade J, Sienski G, Duchek P
  TITLE     Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome 
            Editing in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126.
  PUBMED    25236734
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126."
COMMENT     Sequence Label: pDCC6
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    103..127
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(144..164)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta tgtcacacca
       61 cagaagtaag gttccttcac aaagatcctg ctagcttacg tcaccacttt gtacaagaaa
      121 gctgggtcct attagctggc ctccaccttt ctcttcttct tggggctgtc gcctcccagc
      181 tgagacaggt cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat cagggtggcg
      241 tccagcacct ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg
      301 aaggcggcag gggctcccag attggtcagg gtaaacaggt ggatgatatt cttggcctgc
      361 tctctgatgg gcttatcccg gtgcttgttg taggcggaca gcactttgtc cagattagcg
      421 tcggccagga tcactctctt ggagaactcg ctgatctgct cgatgatctc gtccaggtag
      481 tgcttgtgct gttccacaaa cagctgtttc tgctcattat cctcggggga gcccttcagc
      541 ttctcatagt ggctggccag gtacaggaag ttcacatatt tggagggcag ggccagttcg
      601 tttcccttct gcagttcgcc ggcagaggcc agcattctct tccggccgtt ttccagctcg
      661 aacagggagt acttaggcag cttgatgatc aggtcctttt tcacttcttt gtagcccttg
      721 gcttccagaa agtcgatggg attcttctcg aagctgcttc
//
