LOCUS       sequence-97387-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and 
            U6>sgRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Gokcezade J, Sienski G, Duchek P
  TITLE     Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome 
            Editing in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126.
  PUBMED    25236734
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126."
COMMENT     Sequence Label: pDCC6
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(323..343)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(350..415)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      415..424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtctgtggaa gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat
       61 agcagatccg gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg
      121 cctcggctgt ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt
      181 gccggtcggt gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga
      241 tcacggccca gcccacagag ttggtgccga tgtccaggcc gatgctgtac ttcttgtcgg
      301 ctgctgggac tccgtggata ccgaccttcc gcttcttctt tggggccatc ttatcgtcat
      361 cgtctttgta atcaatatca tgatccttgt agtctccgtc gtggtcctta tagtccatgg
      421 tggcaccggt agagctccga attcactgca gtggtaccgc ctaggtgtat acctccggaa
      481 gcgcgcactc gagattcgaa caagcttatc gagcttggct gcagattgtt tagcttgttc
      541 agctgcgctt gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga
      601 attgaattgt cgctccgtag acgaagcgcc tctatttata ctccggcgct cttttcgcga
      661 acattcgagg cgcgctctct cgaaccaacg agagcagtat gccgtttact gtgtgacaga
      721 gtgagagagc attagtgcag agagggagag acccaaaaag aaaagagaga ataacgaata
      781 acggccagag aaatttctcg agttttcttt ctgccaaaca aatgacctac cacaataacc
      841 ag
//
