LOCUS       sequence-97389-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and 
            U6>sgRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Gokcezade J, Sienski G, Duchek P
  TITLE     Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome 
            Editing in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126.
  PUBMED    25236734
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 
            10.1534/g3.114.014126."
COMMENT     Sequence Label: pDCC6
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(321..396)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(415..814)
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
ORIGIN
        1 gcgcctctat ttatactccg gcgctctttt cgcgaacatt cgaggcgcgc tctctcgaac
       61 caacgagagc agtatgccgt ttactgtgtg acagagtgag agagcattag tgcagagagg
      121 gagagaccca aaaagaaaag agagaataac gaataacggc cagagaaatt tctcgagttt
      181 tctttctgcc aaacaaatga cctaccacaa taaccagttt gttttgggat tctagagcca
      241 tttgtctgca gaattggcgc acgcgctaaa aacggactag ccttatttta acttgctatt
      301 tctagctcta aaacaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
      361 agccttattt taacttgcta tttctagctc taaaacaggt cttctcgaag acccgaagta
      421 ttgaggaaaa catacctata taaatgatca acatcaggaa agagcagttg agaattataa
      481 gaattggcaa atggtcttaa gaaccctctg cttaagattt tcaaaatttc ctttagaatc
      541 aaagtgtcct attgttcgtt tgttgaaaac acagttcgat ttattgacta taataaattg
      601 atagttttaa atatagaggc acgactaaga gagtttggtt ttgttttggt tagggaccaa
      661 aaaaagtata cataacgaat aaaaaaggat ttaagtgcaa atggtaaaaa agtgtcgagt
      721 ttttcttgag ttgattgtgc tgtaatgaga ctctgcattc ggcatttgac tcggcttttc
      781 ctactcgtgc cgtatttcag gctgcaagtc gaaccgctga tccgcctacc tttcacgagt
      841 tgcctgcatt aatgaatcgg ccaacgcgc
//
