LOCUS       sequence-97390-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sigma32 I54N mutant in E. coli to render a heat-shock like
            cellular folding environment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Zhang X, Liu Y, Genereux JC, Nolan C, Singh M, Kelly JW
  TITLE     The Heat-Shock Response Transcriptional Program Enables High-Yield 
            and High-Quality Recombinant Protein Production in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2014 Jul 22.
  PUBMED    25051296
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2014 Jul 22."
COMMENT     Sequence Label: pBAD.Sigma32.I54N
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcttctatta cgcttcaatg gcagcacgca attttttcat cgcgttcttt tccagctggc
       61 gtacacgctc agcggaaacg ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt
      121 cttcgtccag ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccctgcatcg
      181 cgtcggtcag acggtttgcc gcctgctctt cccagttatc atcttcaatg ccgtcggcaa
      241 agttagatga tttatcctgc agatagagca ccggagccat cggctggctg tcggaatcgt
      301 cgtcggaaga caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta
      361 cgtctttgct ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc
      421 agcccagacg ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg
      481 tcgcaacttt gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc
      541 agtgaacggc gaaggagacc aggcgcacac ccacttccgg gttgaaacgg cgcactgctt
      601 tcatcaggcc gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat
      661 aattacgagc aatatgaaca acaaaccg
//
