LOCUS       sequence-97391-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sigma32 I54N mutant in E. coli to render a heat-shock like
            cellular folding environment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Zhang X, Liu Y, Genereux JC, Nolan C, Singh M, Kelly JW
  TITLE     The Heat-Shock Response Transcriptional Program Enables High-Yield 
            and High-Quality Recombinant Protein Production in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2014 Jul 22.
  PUBMED    25051296
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2014 Jul 22."
COMMENT     Sequence Label: pBAD.Sigma32.I54N
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctacaggagg aattaaccat gactgacaaa atgcaaagtt tagctttagc cccagttggc
       61 aacctggatt cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag
      121 cgggcgctgg ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgaac
      181 ctgtctcacc tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca
      241 caggcggatt tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgtttcaac
      301 ccggaagtgg gtgtgcgcct ggtctccttc gccgttcact ggatcaaagc agagatccac
      361 gaatacgttc tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcagcgcaaa
      421 ctgttcttca acctgcgtaa aaccaagcag cgtctgggct ggtttaacca ggatgaagtc
      481 gaaatggtgg cccgtgaact gggcgtaacc agcaaagacg tacgtgagat ggaatcacgt
      541 atggcggcac aggacatgac ctttgacctg tcttccgacg acgattccga cagccagccg
      601 atggctccgg tgctctatct gcaggataaa tcatctaact ttgccgacgg cattgaagat
      661 gataactggg aagagc
//
