LOCUS       sequence-97394-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY cloning compatible shRNA expression vector; H1 RNA gene 
            promoter; Kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-H1
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          435..638
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     595..615
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggattgggat cttataagtt ctgtatgaga ccacagatct gcacactggt atttcggttt
       61 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
      121 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
      181 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
      241 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
      301 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
      361 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
      421 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
      481 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
      541 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
      601 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attagcttgc
      661 ttacatttgc ttctgacaca actgtgttca ctagcaacct caaacagaca ccatggggta
      721 aaggagaaga actttttcac tggagttgtc ccaaattctt gtttgaatta gatggtgatg
      781 ttaat
//
