LOCUS       sequence-97400-e        8829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mammalian expression of ERAS. Upon CRE mediated 
            deletion of ERAS insert, dsRED expression markers is truned on due 
            to concomittant removal of stop cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRE...
FEATURES             Location/Qualifiers
     source          1..8829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    576..710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        724..1107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1112..1389
                     /label=chicken beta-actin promoter
     intron          1390..2406
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2330..2352
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2414..2433
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(2467..2500)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3292..3325)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3331..4008
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(3461..3479)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3511..3531)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3729..3748
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3923..3946
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    join(4049..4623,4624..4626,4627..4635)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4216..4233)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4443..4462
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4636..5235
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4636..4655)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5132..5152
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5316..5333)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5322..5546
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5593..5609)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5593..5609)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5606..5628)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5617..5633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5641..5647,5648..5665,5666..5671))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5686..5707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5766..5965
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5812..5951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5971..6105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             6574..6765
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6759..6781)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6867..7007
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6922..6941
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7022..7039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7193..7781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7273..7292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7952..8743,8744..8812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8575..8594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaccgctttg gaaaaacaaa gactgtattt cctggaaatt aatgtttatt caataaactg
       61 tgtattcagc tatattcaca tagtggtgag gctgaaatga ggcgggaaga ggcggttggg
      121 gcttaattat atcaatttgg gtggccccac agcgcctcca aggcgccagt cctgttttga
      181 caagttgcct ctggaagcct ctcttctttt tctccagagt aagcggaggc caggggcccc
      241 cggcctctgc ttaatactaa aaaaaacagc tgttgtcata gtaatgattg ggtggaaaca
      301 ttccaggcct gggtggagag gctttttgct tcctcttgca aaaccacact gacattccag
      361 gcctgggtgg agaggctttt tgcttcctct tgcaaaacca cactgccctc tggagggcag
      421 ttgcctagca actaattaaa agaggatgtc gcacggccag ctgcggtcag ttagtcactt
      481 cctgcttaac tgacttgaca ttttctattt taagagtcgg gaggaaaatt actgtgttgg
      541 aggccctccg ccatcttctg aagctgaatc gaattaactt gtttattgca gcttataatg
      601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatatcatc
      721 gtcgacattg attattgact agctagttat taatagtaat caattacggg gtcattagtt
      781 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      841 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      901 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      961 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1021 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1081 tacgtattag tcatcgctat taccatgatg gtcgaggtga gccccacgtt ctgcttcact
     1141 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1201 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1261 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1321 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1381 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1441 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1501 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     1561 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     1621 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     1681 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     1741 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     1801 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     1861 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     1921 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     1981 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2041 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2101 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2161 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2221 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2281 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2341 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2401 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
     2461 ctcgacataa cttcgtatag catacattat acgaagttat ctcgaatgga gctgccaaca
     2521 aagcctggca ccttcgacct gggcctggcc acatggagcc cttccttcca gggggaaacc
     2581 caccgggctc aggcacgccg cagggatgtt ggcaggcagc tgcctgagta caaggctgtg
     2641 gtggtgggcg ccagtggcgt gggcaagagt gcgctgacca tccagctgaa ccaccagtgc
     2701 ttcgtggagg accacgaccc caccatccag gattcctact ggaaggagtt gaccctggac
     2761 agtggggact gcattctgaa tgtgctggac acagcagggc aggccatcca tagggccctg
     2821 cgtgaccagt gcctggctgt ctgtgatggt gtgctgggcg tcttcgctct cgatgacccc
     2881 tcgtctctga tccagctgca gcagatatgg gccacctggg gccctcaccc cgcccagccc
     2941 cttgtcctcg tgggcaacaa gtgtgacctt gtgaccactg ctggagatgc tcatgccgct
     3001 gctgcagccc tcgcacacag ctggggggcc cacttcgtgg agacctcggc caaaacacgg
     3061 caaggcgtgg aggaggcctt ttccctgctg gtccatgaga tccagagggt ccaggaggcc
     3121 atggcgaagg agcccatggc aaggtcctgt agggagaaga cccggcacca gaaggccacc
     3181 tgccactgtg gctgctctgt ggcctgaagg tcttggccaa gaaatgtaga cctttcccca
     3241 ggccagggtg acgtcgacga agggcgaatt ggccgcacta gttacttagg tataacttcg
     3301 tatagcatac attatacgaa gttatccacc atggcctcct ccgaggacgt catcaaggag
     3361 ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag
     3421 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3481 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag
     3541 gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc
     3601 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     3661 tcctccctgc aggacggctg cttcatctac aaggtgaagt tcatcggcgt gaacttcccc
     3721 tccgacggcc ccgtaatgca gaagaagact atgggctggg agccctccac cgagcgcctg
     3781 tacccccgcg acggcgtgct gaagggcgag atccacaagg ccctgaagct gaaggacggc
     3841 ggccactacc tggtggagtt caagtccatc tacatggcca agaagcccgt gcagctgccc
     3901 ggctactact acgtggactc caagctggac atcacctccc acaacgagga ctacaccatc
     3961 gtggagcagt acgagcgcgc cgagggccgc caccacctgt tcctgtagac ggccgctcga
     4021 taagcttgat atcgaattcc gccccccccc cctctccctc ccccccccct aacgttactg
     4081 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     4141 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     4201 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     4261 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     4321 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     4381 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     4441 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     4501 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     4561 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     4621 aatatggcca caaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4681 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4741 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4801 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4861 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4921 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4981 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     5041 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     5101 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     5161 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     5221 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     5281 ccccatgcat cgatgatcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     5341 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5401 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5461 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5521 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaacctgca gcccaagctt
     5581 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5641 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5701 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     5761 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5821 ctaactccgc ccagttccgc ccattctccg ccccatgcat ggctgactaa ttttttttat
     5881 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5941 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     6001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcccacg ggtgcgcatg
     6121 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
     6181 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
     6241 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
     6301 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
     6361 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
     6421 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
     6481 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
     6541 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
     6601 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
     6661 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
     6721 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6781 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6841 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6901 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6961 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     7021 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7081 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7141 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7201 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7261 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7321 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7381 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7441 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7501 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7561 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7621 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7681 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7741 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7801 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7861 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7921 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7981 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8041 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8101 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8161 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8221 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     8281 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8341 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8401 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8461 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8521 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8581 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8641 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8701 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8761 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8821 tttttcaat
//
