LOCUS       sequence-97401-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for 
            sgRNA cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..122)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
       61 ggttgattat cgataagctt gatatcgaat tcttaagcgt aatctggaac atcgtatggg
      121 tagtcaccat cttcgagcag tctcaccatg gccccggttt cagagtcaag attgcggata
      181 tagttcataa cgatgttcac atttgtccaa cctccagctt gcataatctc tggtatggag
      241 actcctgccc gcgccatgtc tcgtgcggcc cccaccctag cagaatgacc tgaccaggcg
      301 agatacctct ggccactgtc atccttggct ccgtatatga gtcggtgggt tgcctcaaag
      361 atcccttcaa gggcccgtgt actcaattgg ctggtggcag agggcgcggc gactccgttt
      421 tttctcactc gacagaacaa gtaattatta gggtcgtcag ccacgccgga cactgagatc
      481 catctttcca ccagcttcgt tacgcccagg gacaaggcct tttcaacgcc agcggtgctg
      541 accaatgtct ttgtgcgtcc aatatgaata agcatccggc cgccgtccgt tcgactgatg
      601 tctttcactc tgatccgggc gatctcagct atgcgcagaa gtgtgttata tgcaattccc
      661 agaaatgcga ggttcctgat gtcctggcac cgatcgctgt tctccatcag ggatctgacc
      721 tggtcgaaat cagtccgctc aaaagcgaga gcctgcttag ccctctcacc cgcatctacg
      781 ttttcctttc taattctcct cataacaagg gagactgcgt ttgaatcgct aggccggggg
      841 agcccgctgc gtcgatgcag catattcagt tggcccaagt gctgctggat agtcttgaca
      901 gccagccctc ttgcctggag atacagcagg taatcgcgca cgtcctccgg ctcggcggg
//
