LOCUS       sequence-97403-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry-tagged MitoTrap in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ
  TITLE     Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates 
            kinetochore fiber tension.
  JOURNAL   J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. 
            Epub 2013 Mar 26.
  PUBMED    23532825
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-05-1"; volume: "126(Pt 9):2102-13. doi"; pages: "
            10.1242/jcs.124834. Epub 2013 Mar 26"
COMMENT     Sequence Label: pMito-mCherry-FRB
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      181..190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(332..350)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     600..619
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 gccaccatga agagcttcat tacaaggaac aagacagcca ttttggcaac cgttgctgct
      121 acaggtactg ccatcggtgc ctactattat tacaaccaat tgcaacagga tccaccggtc
      181 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      241 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      301 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      361 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      421 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      481 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      541 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      601 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      661 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      721 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      781 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
      841 tacgaacgcg ccgagggccg ccactccacc gg
//
