LOCUS       sequence-97412-e         310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knocks down endogenous TACC3 and re-expresses knockdown-proof 
            (RNAi-resistant) GFP-tagged human TACC3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Booth DG, Hood FE, Prior IA, Royle SJ
  TITLE     A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by 
            inter-microtubule bridging.
  JOURNAL   EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 
            2011 Feb 4.
  PUBMED    21297582
REFERENCE   2  (bases 1 to 310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.15"; journalName: "EMBO J"; date: "2011-03-2- 
            2"; volume: "30"; issue: "5"; pages: "906-19"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP-shTACC3
FEATURES             Location/Qualifiers
     source          1..310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     41..57
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        77..291
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     212..231
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaacta gtggatcccc
       61 cgggctgcag gaattcgaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag
      121 tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga
      181 caggggagtg gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa
      241 acgtgaaatg tctttggatt tgggaatctt ataagttctg tatgagacca cagatccccg
      301 ttaccggaag
//
