LOCUS       sequence-97413-e         305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A 
            substitution and knocks down endogenous TACC3 via shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Booth DG, Hood FE, Prior IA, Royle SJ
  TITLE     A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by 
            inter-microtubule bridging.
  JOURNAL   EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 
            2011 Feb 4.
  PUBMED    21297582
REFERENCE   2  (bases 1 to 305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.15"; journalName: "EMBO J"; date: "2011-03-2- 
            2"; volume: "30"; issue: "5"; pages: "906-19"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP(S558A)-shTACC3
FEATURES             Location/Qualifiers
     source          1..305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     35..51
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        71..285
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     206..225
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat cccccgggct
       61 gcaggaattc gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac
      121 taggcgggaa cacccagcgc gcgtgcgccc tggcaggaag atggctgtga gggacagggg
      181 agtggcgccc tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga
      241 aatgtctttg gatttgggaa tcttataagt tctgtatgag accacagatc cccgttaccg
      301 gaaga
//
