LOCUS       sequence-97422-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for 
            sgRNA cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             140..196
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             197..217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caagaagttt cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga
       61 tgcacctgat gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga
      121 acaagctagc ggaagcggag ccactaactt ctccctgttg aaacaagcag gggatgtcga
      181 agagaatccc gggccaccca agaagaagag gaaggtgtcc aatctcctga ctgttcacca
      241 gaacctccct gcgctgccag tagatgccac tagcgatgag gtcaggaaaa atctcatgga
      301 tatgtttagg gatagacagg cgttttctga acacacctgg aaaatgctgc ttagcgtgtg
      361 ccgatcctgg gcagcctggt gtaagctgaa caatcgcaaa tggttccccg ccgagccgga
      421 ggacgtgcgc gattacctgc tgtatctcca ggcaagaggg ctggctgtca agactatcca
      481 gcagcacttg ggccaactga atatgctgca tcgacgcagc gggctccccc ggcctagcga
      541 ttcaaacgca gtctcccttg ttatgaggag aattagaaag gaaaacgtag atgcgggtga
      601 gagggctaag caggctctcg cttttgagcg gactgatttc gaccaggtca gatccctgat
      661 ggagaacagc gatcggtgcc aggacatcag gaacctcgca tttctgggaa ttgcatataa
      721 cacacttctg cgcatagctg agatcgcccg gatcagagtg aaagacatca gtcgaacgga
      781 cggcggccgg atgcttattc atattggacg cacaaagaca ttggtcagca ccgctggcgt
      841 tgaaaaggcc ttgtccctgg gcgtaacgaa gctggtggaa agatggatct cagtgtccgg
      901 cgtggctgac gaccctaata attacttg
//
