LOCUS       sequence-97425-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for amplification of a selection cassette that 
            includes two transcription terminator elements, an I-SceI site and a
            modified chloramphenicol resistance gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pT2SCb
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(39..141)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(442..460)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        528..632
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(851..870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
      121 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
      181 gtatcaacag ggacaccagg attaccctgt tatccctagc tgacactgag gatctactta
      241 catactgctc gcgataactg actctgccac tcaagaccaa aaaaaacccc gccctgtcag
      301 gggcggggtt ttttttttgt taggtcagtg acctcttaag agcgaaaaaa ccccgccgaa
      361 gcggggtttt ttgcgggtaa gcgtcactaa cgacattggg ttcagttact taacagaacc
      421 gctgccactc ttgagataag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      481 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      541 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      601 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      661 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      721 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      781 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      841 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      901 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      961 atcttacgga tggcatgaca gtaagagaat t
//
