LOCUS       sequence-97429-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the lambda Red recombinase under control of the 
            arabinose-inducible P(araB) promoter and I-SceI under control of the
            anhydrotetracycline-inducible P(tetA) promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pSLTS
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(74..318)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtctt aaccatggat
       61 tcttcgtctg tttctactgg tattggcaca aacctgattc caatttgagc aaggctatgt
      121 gccatctcga tactcgttct taactcaaca gaagatgctt tgtgcataca gcccctcgtt
      181 tattatttat ctcctcagcc agccgctgtg ctttcagtgg atttcggata acagaaaggc
      241 cgggaaatac ccagcctcgc tttgtaacgg agtagacgaa agtgattgcg cctacccgga
      301 tattatcgtg aggatgcgtc atcgccattg ctccccaaat acaaaaccaa tttcagccag
      361 tgcctcgtcc attttttcga tgaactccgg cacgatctcg tcaaaactcg ccatgtactt
      421 ttcatcccgc tcaatcacga cataatgcag gccttcacgc ttcatacgcg ggtcatagtt
      481 ggcaaagtac caggcatttt ttcgcgtcac ccacatgctg tactgcacct gggccatgta
      541 agctgacttt atggcctcga aaccaccgag ccggaacttc atgaaatccc gggaggtaaa
      601 cgggcatttc agttcaaggc cgttgccgtc actgcataaa ccatcgggag agcaggcggt
      661 acgcatactt tcgtcgcgat agatgatcgg ggattcagta acattcacgc cggaagtgaa
      721 ttcaaacagg gttctggcgt cgttctcgta ctgttttccc caggccagtg ctttagcgtt
      781 aacttccgga gccacaccgg tgcaaacctc agcaagcagg gtgtggaagt aggacatttt
      841 catgtcaggc cacttctttc cggagcgggg ttttgctatc acgttgtgaa cttctgaagc
      901 ggtgatgacg ccgagccgta atttgtgcca cgcatcatcc ccctgttcga cagctctcac
      961 atcgatcccg gtacgctgca gga
//
