LOCUS       sequence-97431-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry-tagged MitoTrap in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ
  TITLE     Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates 
            kinetochore fiber tension.
  JOURNAL   J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. 
            Epub 2013 Mar 26.
  PUBMED    23532825
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-05-1"; volume: "126(Pt 9):2102-13. doi"; pages: "
            10.1242/jcs.124834. Epub 2013 Mar 26"
COMMENT     Sequence Label: pMito-mCherry-FRB
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(127..396)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             complement(130..408)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             complement(427..453)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(729..748)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagattatca
      121 gccggcctgc tttgagattc gtcggaacac atgataatag aggtcccagg cttgggtgag
      181 gtccttgaca ttccctgatt tcatgtactt cctgcaccac tcttgggcct ccattaaatc
      241 tcgaccatag gcctgattaa aggatgtttc cttcagagtc tgggggcccc gttccatcat
      301 agcatgcaag ggctccagca cctcaaacat gcctttcacg ttcctttccc caaagtacaa
      361 acgagatgcc tcttccaggc cttcatgcca catctcatgc cagaggatgg ccactcgtgc
      421 ggagcctgcg tagtcgggca cgtcgtaggg gtaagtgtac agctcgtcca tgccgccggt
      481 ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga
      541 ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc
      601 cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg
      661 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca
      721 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt
      781 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
      841 gccgtcctcg aagttcatca cgcgctc
//
