LOCUS       sequence-97435-e         316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knocks down TACC3 in mammalian cells via shRNA and co-expresses 
            GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Booth DG, Hood FE, Prior IA, Royle SJ
  TITLE     A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by 
            inter-microtubule bridging.
  JOURNAL   EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 
            2011 Feb 4.
  PUBMED    21297582
REFERENCE   2  (bases 1 to 316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.15"; journalName: "EMBO J"; date: "2011-03-2- 
            2"; volume: "30"; issue: "5"; pages: "906-19"
COMMENT     Sequence Label: pBrain-GFP-shTACC3
FEATURES             Location/Qualifiers
     source          1..316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..63
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        83..297
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     218..237
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg
       61 atcccccggg ctgcaggaat tcgaacgctg acgtcatcaa cccgctccaa ggaatcgcgg
      121 gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc cctggcagga agatggctgt
      181 gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca
      241 ccataaacgt gaaatgtctt tggatttggg aatcttataa gttctgtatg agaccacaga
      301 tccccgttac cggaag
//
