LOCUS       sequence-97436-e        8210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the human HIPK3 exon 2 circular RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Liang D, Wilusz JE
  TITLE     Short intronic repeat sequences facilitate circular RNA production.
  JOURNAL   Genes Dev. 2014 Oct 3. pii: gad.251926.114.
  PUBMED    25281217
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2014 Oct 3. pii: gad.251926.114."
COMMENT     Sequence Label: pcDNA3.1(+) HIPK3 Sense
FEATURES             Location/Qualifiers
     source          1..8210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3804..3821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3810..4034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4080..4508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4167..4186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4377..4398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4517..4537)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4522..4851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4702..4837
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4764..4783
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4918..5712
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4972..4991)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5582..5601
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5886..6007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5923..5942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5977..5996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6056..6072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6056..6072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6069..6091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6080..6096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6104..6110,6111..6128,6129..6134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6149..6170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6287..6304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6458..7043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6538..6557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7214..8005,8006..8074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7837..7856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8075..8179)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgaa
      961 tttggtaata gaatgtttta cttttgtcaa atgtgtacag ttaaaggatc tgtacttttt
     1021 tttgagacag agtcttgctc tgtctcacag gctgaagtgc agtggtgcaa tctcggctca
     1081 ctgcaacctc cacctcccag gttcaagcga ttctcctccc tcagcctccc gagtagctgg
     1141 gaccacaggc atgcaccacc atccccagct aatttttgca ttattagtag agttgggatt
     1201 tcttcaccgt gttggccagg ctggtcttgg actcctgacc tcaagtggtc caactgcctc
     1261 agcctctcaa agtgctagga ttacagggat ctatactttt cttttgaggg aaaatgttgg
     1321 caccgtttct agggcatatt ggccatttca gcttctcagt aaatatttgt taagtaatta
     1381 aatgcacttg attctttatt cttagccttt taacgcaata ctcagaatag ctgaagcacc
     1441 aattaactga aatggagata ttataaagat agttatcttc tccaagggaa aaaatcgtct
     1501 tcatggaaat taattacttt tttacaaatt gtgaatttga cccttaagag ttttcttcct
     1561 gatatttaaa attgaaaaaa aaattgttga cattaatatt tcttctttcc ttttttttct
     1621 tttccttttt tttttttttt ttgcaggtat ggcctcacaa gtcttggtct acccaccata
     1681 tgtttatcaa actcagtcaa gtgccttttg tagtgtgaag aaactcaaag tagagccaag
     1741 cagttgtgta ttccaggaaa gaaactatcc acggacctat gtgaatggta gaaactttgg
     1801 aaattctcat cctcccacta agggtagtgc ttttcagaca aagataccat ttaatagacc
     1861 tcgaggacac aacttttcat tgcagacaag tgctgttgtt ttgaaaaaca ctgcaggtgc
     1921 tacaaaggtc atagcagctc aggcacagca agctcacgtg caggcacctc agattggggc
     1981 gtggcgaaac agattgcatt tcctagaagg cccccagcga tgtggattga agcgcaagag
     2041 tgaggagttg gataatcata gcagcgcaat gcagattgtc gatgaattgt ccatacttcc
     2101 tgcaatgttg caaaccaaca tgggaaatcc agtgacagtt gtgacagcta ccacaggatc
     2161 aaaacagaat tgtaccactg gagaaggtga ctatcagtta gtacagcatg aagtcttatg
     2221 ctccatgaaa aatacttacg aagtccttga ttttcttggt cgaggcacgt ttggccaggt
     2281 agttaaatgc tggaaaagag ggacaaatga aattgtagca atcaaaattt tgaagaatca
     2341 tccttcttat gcccgtcaag gtcaaataga agtgagcata ttagcaaggc tcagtactga
     2401 aaatgctgat gaatataact ttgtacgagc ttatgaatgc tttcagcacc gtaaccatac
     2461 ttgtttagtc tttgagatgc tggaacaaaa cttgtatgac tttctgaaac aaaataaatt
     2521 tagtcccctg ccactaaaag tgattcggcc cattcttcaa caagtggcca ctgcactgaa
     2581 aaaattgaaa agtcttggtt taattcatgc tgatctcaag ccagagaata ttatgttggt
     2641 ggatcctgtt cggcagcctt acagggttaa agtaatagac tttgggtcgg ccagtcatgt
     2701 atcaaagact gtttgttcaa catatctaca atctcggtac tacaggtagg taacaactcc
     2761 atactttttg gttgtttatt aatgtgaaat ttctgctaaa tgaaatactt ttgtgtgtgt
     2821 ttgtggtaga agagaccact tcagttaaat aaggaaatca agagaggatc aatttaggtt
     2881 cgttttaaag agattaaaaa aaatcaagac ataaaatcta cccaagcagg atagaaatct
     2941 ccactgcaaa gttccatgcc aaagacatct ggttattttt atttttaatg gaagacttga
     3001 aggaatgata ggtgattaat aatgatcaaa cagaagtctt taaatgttgg aaagtattta
     3061 cattaatctt tgtatatatc attgggcatt ttagcacttg agagaaatag tttattaaag
     3121 atataatcaa tcatatgtaa ctgaacattt agaaaaatta tatacaggtt tgagtagccc
     3181 ttatctgaaa cttttggggc cagaagtgtt ttggattcca gatttttccg gattttggaa
     3241 tatttgcact gccaactagt taagcacccc caaatctgaa aattcgtttc ctttgagtgt
     3301 catgtcaatg cccaaaaagt ttcagatatt tggatttgag atgctcaacc tgtataagga
     3361 ttcagaaagt tattctgatt aatgatttta agattcagat atacagccgg gtgcagtggc
     3421 tcatgcctgt aatccctgca cttagggagg ctgaggcggg tggatgacct gaggttagga
     3481 gttcaagacc agcctggcca acatggcgaa acccccatct ctactaaaaa taacaaaaat
     3541 tagctgggtg tggtggtggg tgtctataat cccagcaact tgggaggctg aggcaggaga
     3601 atcacttgaa cccaggagat ggaggttgca gtgagccgag atcatgccat tgcactccag
     3661 cctgggccac aagagcaaaa ctctgtctca aaaaaacaaa acaaaacaca aatatacttc
     3721 tttggctccc gtgactcttt aggtcacctg atcataaccg cggccgctcg agtctagagg
     3781 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3841 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     3901 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     3961 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4021 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4081 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4141 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4201 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4261 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4321 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4381 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4441 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4501 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4561 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4621 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4681 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4741 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4801 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4861 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     4921 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4981 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5041 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5101 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5161 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5221 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5281 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5341 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5401 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5461 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5521 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5581 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5641 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5701 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5761 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5821 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5881 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5941 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6001 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6061 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6121 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6241 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6301 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6421 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6481 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6541 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6601 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6661 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6721 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6781 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6841 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6901 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6961 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta
     7021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
