LOCUS       sequence-97437-e        8203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative Control for the pcDNA3.1(+) HIPK3 Sense plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Liang D, Wilusz JE
  TITLE     Short intronic repeat sequences facilitate circular RNA production.
  JOURNAL   Genes Dev. 2014 Oct 3. pii: gad.251926.114.
  PUBMED    25281217
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2014 Oct 3. pii: gad.251926.114."
COMMENT     Sequence Label: pcDNA3.1(+) HIPK3 Antisense
FEATURES             Location/Qualifiers
     source          1..8203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3797..3814)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3803..4027
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4073..4501
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4160..4179)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4370..4391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4510..4530)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4515..4844
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4695..4830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4757..4776
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4911..5705
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4965..4984)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5575..5594
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5879..6000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5916..5935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5970..5989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6049..6065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6049..6065)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6062..6084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6073..6089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6097..6103,6104..6121,6122..6127))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6142..6163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6280..6297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6451..7036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6531..6550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7207..7998,7999..8067))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7830..7849
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8068..8172)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcggt
      961 tatgatcagg tgacctaaag agtcacggga gccaaagaag tatatttgtg ttttgttttg
     1021 tttttttgag acagagtttt gctcttgtgg cccaggctgg agtgcaatgg catgatctcg
     1081 gctcactgca acctccatct cctgggttca agtgattctc ctgcctcagc ctcccaagtt
     1141 gctgggatta tagacaccca ccaccacacc cagctaattt ttgttatttt tagtagagat
     1201 gggggtttcg ccatgttggc caggctggtc ttgaactcct aacctcaggt catccacccg
     1261 cctcagcctc cctaagtgca gggattacag gcatgagcca ctgcacccgg ctgtatatct
     1321 gaatcttaaa atcattaatc agaataactt tctgaatcct tatacaggtt gagcatctca
     1381 aatccaaata tctgaaactt tttgggcatt gacatgacac tcaaaggaaa cgaattttca
     1441 gatttggggg tgcttaacta gttggcagtg caaatattcc aaaatccgga aaaatctgga
     1501 atccaaaaca cttctggccc caaaagtttc agataagggc tactcaaacc tgtatataat
     1561 ttttctaaat gttcagttac atatgattga ttatatcttt aataaactat ttctctcaag
     1621 tgctaaaatg cccaatgata tatacaaaga ttaatgtaaa tactttccaa catttaaaga
     1681 cttctgtttg atcattatta atcacctatc attccttcaa gtcttccatt aaaaataaaa
     1741 ataaccagat gtctttggca tggaactttg cagtggagat ttctatcctg cttgggtaga
     1801 ttttatgtct tgattttttt taatctcttt aaaacgaacc taaattgatc ctctcttgat
     1861 ttccttattt aactgaagtg gtctcttcta ccacaaacac acacaaaagt atttcattta
     1921 gcagaaattt cacattaata aacaaccaaa aagtatggag ttgttaccta cctgtagtac
     1981 cgagattgta gatatgttga acaaacagtc tttgatacat gactggccga cccaaagtct
     2041 attactttaa ccctgtaagg ctgccgaaca ggatccacca acataatatt ctctggcttg
     2101 agatcagcat gaattaaacc aagacttttc aattttttca gtgcagtggc cacttgttga
     2161 agaatgggcc gaatcacttt tagtggcagg ggactaaatt tattttgttt cagaaagtca
     2221 tacaagtttt gttccagcat ctcaaagact aaacaagtat ggttacggtg ctgaaagcat
     2281 tcataagctc gtacaaagtt atattcatca gcattttcag tactgagcct tgctaatatg
     2341 ctcacttcta tttgaccttg acgggcataa gaaggatgat tcttcaaaat tttgattgct
     2401 acaatttcat ttgtccctct tttccagcat ttaactacct ggccaaacgt gcctcgacca
     2461 agaaaatcaa ggacttcgta agtatttttc atggagcata agacttcatg ctgtactaac
     2521 tgatagtcac cttctccagt ggtacaattc tgttttgatc ctgtggtagc tgtcacaact
     2581 gtcactggat ttcccatgtt ggtttgcaac attgcaggaa gtatggacaa ttcatcgaca
     2641 atctgcattg cgctgctatg attatccaac tcctcactct tgcgcttcaa tccacatcgc
     2701 tgggggcctt ctaggaaatg caatctgttt cgccacgccc caatctgagg tgcctgcacg
     2761 tgagcttgct gtgcctgagc tgctatgacc tttgtagcac ctgcagtgtt tttcaaaaca
     2821 acagcacttg tctgcaatga aaagttgtgt cctcgaggtc tattaaatgg tatctttgtc
     2881 tgaaaagcac tacccttagt gggaggatga gaatttccaa agtttctacc attcacatag
     2941 gtccgtggat agtttctttc ctggaataca caactgcttg gctctacttt gagtttcttc
     3001 acactacaaa aggcacttga ctgagtttga taaacatatg gtgggtagac caagacttgt
     3061 gaggccatac ctgcaaaaaa aaaaggaaaa gaaaaaaaag gaaagaagaa atattaatgt
     3121 caacaatttt tttttcaatt ttaaatatca ggaagaaaac tcttaagggt caaattcaca
     3181 atttgtaaaa aagtaattaa tttccatgaa gacgattttt tcccttggag aagataacta
     3241 tctttataat atctccattt cagttaattg gtgcttcagc tattctgagt attgcgttaa
     3301 aaggctaaga ataaagaatc aagtgcattt aattacttaa caaatattta ctgagaagct
     3361 gaaatggcca atatgcccta gaaacggtgc caacattttc cctcaaaaga aaagtataga
     3421 tccctgtaat cctagcactt tgagaggctg aggcagttgg accacttgag gtcaggagtc
     3481 caagaccagc ctggccaaca cggtgaagaa atcccaactc tactaataat gcaaaaatta
     3541 gctggggatg gtggtgcatg cctgtggtcc cagctactcg ggaggctgag ggaggagaat
     3601 cgcttgaacc tgggaggtgg aggttgcagt gagccgagat tgcaccactg cacttcagcc
     3661 tgtgagacag agcaagactc tgtctcaaaa aaaagtacag atcctttaac tgtacacatt
     3721 tgacaaaagt aaaacattct attaccaaat tcgcggccgc tcgagtctag agggcccgtt
     3781 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3841 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3901 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3961 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4021 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     4081 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4141 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4201 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4261 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4381 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4441 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4501 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4561 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4621 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4801 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4861 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4921 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4981 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5041 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5101 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5161 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5221 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5281 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5341 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5401 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5461 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5521 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5581 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5641 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5701 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5761 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5821 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6001 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     6061 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6121 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6181 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6241 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6961 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     7021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8161 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
