LOCUS       sequence-97445-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of SV40 large T antigen and mCherry red 
            fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, 
            Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS
  TITLE     Modeling pathogenesis of primary liver cancer in lineage-specific 
            mouse cell types.
  JOURNAL   Gastroenterology. 2013 Jul;145(1):221-31. doi: 
            10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19.
  PUBMED    23523670
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.03.013"; journalName: "Gastroenterology"; 
            date: "2013-07"; volume: "145"; issue: "1"; pages: "221-31"
COMMENT     Sequence Label: pRRLsin-SV40 T antigen-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             133..153
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
       61 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
      121 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
      181 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
      241 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
      301 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
      361 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
      421 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatccattt
      481 tctgttattg aggaaagttt gccaggtggg ttaaaggagc atgattttaa tccagaagaa
      541 gcagaggaaa ctaaacaagt gtcctggaag cttgtaacag agtatgcaat ggaaacaaaa
      601 tgtgatgatg tgttgttatt gcttgggatg tacttggaat ttcagtacag ttttgaaatg
      661 tgtttaaaat gtattaaaaa agaacagccc agccactata agtaccatga aaagcattat
      721 gcaaatgctg ctatatttgc tgacagcaaa aaccaaaaaa ccatatgcca acaggctgtt
      781 gatactgttt tagctaaaaa gcgggttgat agcctacaat taactagaga acaaatgtta
      841 acaaacagat ttaatgatct tttggatagg atggatataa tgtttggttc tacaggctct
      901 gctgacata
//
