LOCUS       sequence-97450-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: BACE2_pCSdest
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgggcgc actggcccgg gcgctgctgc
      181 tgcctctgct ggcccagtgg ctcctgcgcg ccgccccgga gctggccccc gcgcccttca
      241 cgctgcccct ccgggtggcc gcggccacga accgcgtagt tgcgcccacc ccgggacccg
      301 ggacccctgc cgagcgccac gccgacggct tggcgctcgc cctggagcct gccctggcgt
      361 cccccgcggg cgccgccaac ttcttggcca tggtagacaa cctgcagggg gactctggcc
      421 gcggctacta cctggagatg ctgatcggga cccccccgca gaagctacag attctcgttg
      481 acactggaag cagtaacttt gccgtggcag gaaccccgca ctcctacata gacacgtact
      541 ttgacacaga gaggtctagc acataccgct ccaagggctt tgacgtcaca gtgaagtaca
      601 cacaaggaag ctggacgggc ttcgttgggg aagacctcgt caccatcccc aaaggcttca
      661 atacttcttt tcttgtcaac attgccacta tttttgaatc agagaatttc tttttgcctg
      721 ggattaaatg gaatggaata cttggcctag cttatgccac acttgcc
//
