LOCUS       sequence-97456-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP with robust performance in protein fusions and useful as FRET 
            acceptor for Clover in a FRET pair that offers bright fluorescence, 
            dynamic range, and photostability while limiting emissions overlap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pcDNA3-mRuby2
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..721)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             complement(join(728..742,743..751))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agaattctta cttgtacagc tcgtccatcc caccaccaag cccggcgaac ttggcaactg
       61 cgtgttcgcg ttgtactacg aacatttcat tgtcactttc ctctaacctt tccaggcggt
      121 gatcaacggc atggataccg ggcatcttga tgttcccgac ggtctttttt gacctgtaag
      181 ttgttacgaa agagcaagac agatggccac caccatcaac tttcagtgcc atatgagtgt
      241 atcccctcag accaccatct gctggataca tcatctctgt attaggctcc caacccttgg
      301 tcttcttctg catcacggga ccattggagg gaaagtttac ccctctgact tggacgtggt
      361 aaacgagaca gccatcctca aggctggtgt cctgcatgac ggtgacgact ccaccatctt
      421 cgtatctcgt aactctttcc caagtaaaac cctcaggaaa ggactgttta aagaaatcag
      481 gaatgccttt cgggtacttg ataaaagtac ggctgccata catgaacgac gtggcaagaa
      541 tgtcaaaggc aaatggcagg ggtcctccct cgatgacttt gatcctcatg gtttgagttc
      601 ccatgtacgg attgccttct ccttcacctg tgcatttgaa ttggtggccg ttgaccgaac
      661 cttccatgac caccttcata cgcatatttt ccttgatcag ctcttcgccc ttagacacca
      721 tcggatcctt atcgtcatcg tcgtacagat cccga
//
