LOCUS       sequence-97458-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase from the Cbh promoter and one U6-driven 
            sgRNA. AAV backbone with SapI spacer for sgRNA cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA...
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..83
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtttattacc tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca
       61 tgcccaagaa gaagaggaag gtgtccaatc tcctgactgt tcaccagaac ctccctgcgc
      121 tgccagtaga tgccactagc gatgaggtca ggaaaaatct catggatatg tttagggata
      181 gacaggcgtt ttctgaacac acctggaaaa tgctgcttag cgtgtgccga tcctgggcag
      241 cctggtgtaa gctgaacaat cgcaaatggt tccccgccga gccggaggac gtgcgcgatt
      301 acctgctgta tctccaggca agagggctgg ctgtcaagac tatccagcag cacttgggcc
      361 aactgaatat gctgcatcga cgcagcgggc tcccccggcc tagcgattca aacgcagtct
      421 cccttgttat gaggagaatt agaaaggaaa acgtagatgc gggtgagagg gctaagcagg
      481 ctctcgcttt tgagcggact gatttcgacc aggtcagatc cctgatggag aacagcgatc
      541 ggtgccagga catcaggaac ctcgcatttc tgggaattgc atataacaca cttctgcgca
      601 tagctgagat cgcccggatc agagtgaaag acatcagtcg aacggacggc ggccggatgc
      661 ttattcatat tggacgcaca aagacattgg tcagcaccgc tggcgttgaa aaggccttgt
      721 ccctgggcgt aacgaagctg gtggaaagat ggatctcagt gtccggcgtg gctgacgacc
      781 ctaataatta cttgttctgt cgagtgagaa aaa
//
