LOCUS       sequence-97461-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and KASH-tagged EGFP from the hSyn 
            promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for 
            sgRNA cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..72
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaggagtcgt gtcgtgcctg agagcgcagt cgagaaggta ccgccaccat gcccaagaag
       61 aagaggaagg tgtccaatct cctgactgtt caccagaacc tccctgcgct gccagtagat
      121 gccactagcg atgaggtcag gaaaaatctc atggatatgt ttagggatag acaggcgttt
      181 tctgaacaca cctggaaaat gctgcttagc gtgtgccgat cctgggcagc ctggtgtaag
      241 ctgaacaatc gcaaatggtt ccccgccgag ccggaggacg tgcgcgatta cctgctgtat
      301 ctccaggcaa gagggctggc tgtcaagact atccagcagc acttgggcca actgaatatg
      361 ctgcatcgac gcagcgggct cccccggcct agcgattcaa acgcagtctc ccttgttatg
      421 aggagaatta gaaaggaaaa cgtagatgcg ggtgagaggg ctaagcaggc tctcgctttt
      481 gagcggactg atttcgacca ggtcagatcc ctgatggaga acagcgatcg gtgccaggac
      541 atcaggaacc tcgcatttct gggaattgca tataacacac ttctgcgcat agctgagatc
      601 gcccggatca gagtgaaaga catcagtcga acggacggcg gccggatgct tattcatatt
      661 ggacgcacaa agacattggt cagcaccgct ggcgttgaaa aggccttgtc cctgggcgta
      721 acgaagctgg tggaaagatg gatctcagtg tccggcgtgg ctgacgaccc taataattac
      781 ttgttctgtc gagtgagaaa aaacggagtc gccgcgccct ctgccaccag ccaattgagt
      841 acacgggccc ttgaagggat ctttgaggca acccacc
//
