LOCUS       sequence-97468-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A 
            substitution and knocks down endogenous TACC3 via shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Booth DG, Hood FE, Prior IA, Royle SJ
  TITLE     A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by 
            inter-microtubule bridging.
  JOURNAL   EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 
            2011 Feb 4.
  PUBMED    21297582
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.15"; journalName: "EMBO J"; date: "2011-03-2- 
            2"; volume: "30"; issue: "5"; pages: "906-19"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP(S558A)-shTACC3
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
       61 ggtaccgcgg gcccgggtat gagtctgcag gtcttaaacg acaaaaatgt cagcaatgaa
      121 aaaaatacag aaaattgcga cttcctgttt tcgccaccag aagttacagg taggtcatct
      181 gttcttcgtg tgtcacagaa agaaaatgtg ccacccaaga acctggccaa agctatgaag
      241 gtgacttttc agacacctct gcgggatcca cagacgcaca ggattctaag tcctagcatg
      301 gccagcaaac ttgaggctcc tttcactcag gatgacaccc ttggactgga aaactcacac
      361 ccggtctgga cacagaaaga gaaccaacag ctcatcaagg aagtggatgc caaaactact
      421 catggaattc tacagaaacc agtggaggct gacaccgacc tcctggggga tgcaagccca
      481 gcctttggga gtggcagctc cagcgagtct ggcccaggtg ccctggctga cctggactgc
      541 tcaagctctt cccagagccc aggaagttct gagaaccaaa tggtgtctcc aggaaaagtg
      601 tctggcagcc ctgagcaagc cgtggaggaa aaccttagtt cctattcctt agacagaaga
      661 gtgacacccg cctctgagac cctagaagac ccttgcagga cagagtccca gcacaaagcg
      721 gagactccgc acggagccga ggaagaatgc aaagcggaga ctccgcacgg agccgaggag
      781 gaatgccggc acggtggggt ctgtgctccc gcagcagtgg ccacttcgcc tcctggtgca
      841 atccctaagg aagcctgcgg aggagcaccc ctgcagggtc tgcctggcga agccctg
//
