LOCUS       sequence-97473-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MitoTrap tagged with photo-activatable GFP in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ
  TITLE     Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates 
            kinetochore fiber tension.
  JOURNAL   J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. 
            Epub 2013 Mar 26.
  PUBMED    23532825
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-05-1"; volume: "126(Pt 9):2102-13. doi"; pages: "
            10.1242/jcs.124834. Epub 2013 Mar 26"
COMMENT     Sequence Label: pMito-PAGFP-FRB.
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(138..407)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             complement(141..419)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             complement(438..464)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(468..716)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSHQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELY"
     primer_bind     complement(507..528)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     856..875
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctgcataac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      121 ctagattatc agccggcctg ctttgagatt cgtcggaaca catgataata gaggtcccag
      181 gcttgggtga ggtccttgac attccctgat ttcatgtact tcctgcacca ctcttgggcc
      241 tccattaaat ctcgaccata ggcctgatta aaggatgttt ccttcagagt ctgggggccc
      301 cgttccatca tagcatgcaa gggctccagc acctcaaaca tgcctttcac gttcctttcc
      361 ccaaagtaca aacgagatgc ctcttccagg ccttcatgcc acatctcatg ccagaggatg
      421 gccactcgtg cggagcctgc gtagtcgggc acgtcgtagg ggtaagtgta cagctcgtcc
      481 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
      541 tcgttggggt ctttgctcag cttggactgg tggctcaggt agtggttgtc gggcagcagc
      601 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
      661 tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcc
      721 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
      781 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      841 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga a
//
