LOCUS       sequence-97476-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knocks down endogenous TACC3 and expresses RNAi-resistant human 
            TACC3 tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ
  TITLE     Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates 
            kinetochore fiber tension.
  JOURNAL   J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. 
            Epub 2013 Mar 26.
  PUBMED    23532825
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-05-1"; volume: "126(Pt 9):2102-13. doi"; pages: "
            10.1242/jcs.124834. Epub 2013 Mar 26"
COMMENT     Sequence Label: pBrain-GFP-FKBP-TACC3-shTACC3
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(58..60,61..381)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 gagctgtacc gaatcgatgt gaatgggggc cctgaacccg ccaagaacct gtacaccatg
       61 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactggaca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc taaaactgga acggtacaag tccggactca gatctcgagc tcaagcttcg
      421 aattctgcag tcgacggtac cgcgggcccg ggtatgagtc tgcaggtctt aaacgacaaa
      481 aatgtcagca atgaaaaaaa tacagaaaat tgcgacttcc tgttttcgcc accagaagtt
      541 acaggtaggt catctgttct tcgtgtgtca cagaaagaaa atgtgccacc caagaacctg
      601 gccaaagcta tgaaggtgac ttttcagaca cctctgcggg atccacagac gcacaggatt
      661 ctaagtccta gcatggccag caaacttgag gctcctttca ctcaggatga cacccttgga
      721 ctggaaa
//
