LOCUS       sequence-97477-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For use in light-induced protein inactivation through the 
            interaction with CRY2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD
  TITLE     Reversible protein inactivation by optogenetic trapping in cells.
  JOURNAL   Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 
            2014 May 4.
  PUBMED    24793453
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2940"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "633-6"
COMMENT     Sequence Label: pCMV-CIB1-mRFP1-MP
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..537
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     regulatory      550..559
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(686..704)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgccaccatg aatggagcta taggaggtga ccttttgctc
       61 aattttcctg acatgtcggt cctagagcgc caaagggctc acctcaagta cctcaatccc
      121 acctttgatt ctcctctcgc cggcttcttt gccgattctt caatgattac cggcggcgag
      181 atggacagct atctttcgac tgccggtttg aatcttccga tgatgtacgg tgagacgacg
      241 gtggaaggtg attcaagact ctcaatttcg ccggaaacga cgcttgggac tggaaatttc
      301 aaggcagcga agtttgatac agagactaag gattgtaatg aggcggcgaa gaagatgacg
      361 atgaacagag atgacctagt agaagaagga gaagaagaga agtcgaaaat aacagagcaa
      421 aacaatggga gcacaaaaag catcaagaag atgaaacaca aagccaagaa agaagagaac
      481 aatttctcta atgattcatc taaagtgacg aaggaattgg agaaaacgga ttatattcat
      541 gtaccggtcg ccaccatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag
      601 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      661 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      721 ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac
      781 cccgccgaca tccccgacta cttgaagctg tccttccccg a
//
