LOCUS       sequence-97478-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For use in light-induced protein inactivation through the 
            interaction with CRY2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD
  TITLE     Reversible protein inactivation by optogenetic trapping in cells.
  JOURNAL   Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 
            2014 May 4.
  PUBMED    24793453
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2940"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "633-6"
COMMENT     Sequence Label: pCMV-CIB1-mCerulean-MP
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..539
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     regulatory      552..561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(603..624)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(864..883)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgccacca tgaatggagc tataggaggt gaccttttgc
       61 tcaattttcc tgacatgtcg gtcctagagc gccaaagggc tcacctcaag tacctcaatc
      121 ccacctttga ttctcctctc gccggcttct ttgccgattc ttcaatgatt accggcggcg
      181 agatggacag ctatctttcg actgccggtt tgaatcttcc gatgatgtac ggtgagacga
      241 cggtggaagg tgattcaaga ctctcaattt cgccggaaac gacgcttggg actggaaatt
      301 tcaaggcagc gaagtttgat acagagacta aggattgtaa tgaggcggcg aagaagatga
      361 cgatgaacag agatgaccta gtagaagaag gagaagaaga gaagtcgaaa ataacagagc
      421 aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca caaagccaag aaagaagaga
      481 acaatttctc taatgattca tctaaagtga cgaaggaatt ggagaaaacg gattatattc
      541 atgtaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      601 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      661 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      721 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct
      781 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      841 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      901 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc a
//
