LOCUS       sequence-97485-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knocks down endogenous TACC3 and expresses RNAi-resistant human 
            TACC3 tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ
  TITLE     Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates 
            kinetochore fiber tension.
  JOURNAL   J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. 
            Epub 2013 Mar 26.
  PUBMED    23532825
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-05-1"; volume: "126(Pt 9):2102-13. doi"; pages: "
            10.1242/jcs.124834. Epub 2013 Mar 26"
COMMENT     Sequence Label: pBrain-GFP-FKBP-TACC3-shTACC3
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..61
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     45..61
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        81..295
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     216..235
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(368..384)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(369..385)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(413..431)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(452..468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(452..468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(465..487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    476..492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(500..506,507..524,525..530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    545..566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 actcactata gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat
       61 cccccgggct gcaggaattc gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc
      121 ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc tggcaggaag atggctgtga
      181 gggacagggg agtggcgccc tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc
      241 ataaacgtga aatgtctttg gatttgggaa tcttataagt tctgtatgag accacagatc
      301 cccgttaccg gaagatcgtc tgttcaagag acagacgatc ttccggtaac tttttggaaa
      361 agcttatcga taccgtcgac ctcgaggggg ggcccggtac cagcttttgt tccctttagt
      421 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      481 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      541 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      601 gaaacctgtc gtgccagata gtaatcaatt acggggtcat tagttcatag cccatatatg
      661 gagttccgcg ttacataact tacggtaaat ggcc
//
