LOCUS       sequence-97502-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Alberts AS
  TITLE     Identification of a carboxyl-terminal diaphanous-related formin 
            homology protein autoregulatory domain.
  JOURNAL   J Biol Chem. 2001 Jan 26. 276(4):2824-30.
  PUBMED    11035012
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Jan 26. 276(4):2824-30."
COMMENT     Sequence Label: pEGFPm-DAD
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..86
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(168..196)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 attagcttgc ttacatttgc ttctgacaca actgtgttca ctagcaacct caaacagaca
       61 ccatgccgca tcaccaccat catcacgcca tgccggagca gaagctgata tccgaggagg
      121 acctggccat ggccggatct agtaaaggag aagaactttt cactggagtt gtcccaattc
      181 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      241 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      301 ttccatggcc aacacttgtc actactctca cttatggtgt tcaatgcttt tcaagatacc
      361 cagatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga tacgtgcagg
      421 agaggaccat cttcttcaag gacgacggga actacaagac acgtgctgaa gtcaagtttg
      481 agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag gaggacggaa
      541 acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac atcatggccg
      601 acaagcaaaa gaacggcatc aaagccaact tcaagacccg ccacaacatc gaagacggcg
      661 gcgtgcaact cgctgatcat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      721 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaa
//
