LOCUS       sequence-9754-ex       10130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac vector with tet inducible mSox2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen
            R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A
  TITLE     piggyBac transposition reprograms fibroblasts to induced pluripotent
            stem cells.
  JOURNAL   Nature. 2009 Apr 9;458(7239):766-70. doi: 10.1038/nature07863
  PUBMED    19252478
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2009-04-9- 9"; volume: "458"; issue: "7239"; pages:
            "766-70"
COMMENT     Sequence Label: PB-TET-mSox2
FEATURES             Location/Qualifiers
     source          1..10130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2533..2557)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2775..2792)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2924..2943
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3155..3175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3185..6226
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             6251..7054
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6314..6333)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6924..6943
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7086..7103)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7092..7316
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7755..7789
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7939..7957)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7978..7994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7978..7994)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7991..8013)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8002..8018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8026..8032,8033..8050,8051..8056))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8071..8092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8209..8226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8380..8968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8460..8479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9139..9930,9931..9999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9762..9781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10000..10104)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg cttgtataac atgatggaga cggagctgaa gccgccgggc ccgcagcaag
     1621 cttcgggggg cggcggcgga ggaggcaacg ccacggcggc ggcgaccggc ggcaaccaga
     1681 agaacagccc ggaccgcgtc aagaggccca tgaacgcctt catggtatgg tcccgggggc
     1741 agcggcgtaa gatggcccag gagaacccca agatgcacaa ctcggagatc agcaagcgcc
     1801 tgggcgcgga gtggaaactt ttgtccgaga ccgagaagcg gccgttcatc gacgaggcca
     1861 agcggctgcg cgctctgcac atgaaggagc acccggatta taaataccgg ccgcggcgga
     1921 aaaccaagac gctcatgaag aaggataagt acacgcttcc cggaggcttg ctggcccccg
     1981 gcgggaacag catggcgagc ggggttgggg tgggcgccgg cctgggtgcg ggcgtgaacc
     2041 agcgcatgga cagctacgcg cacatgaacg gctggagcaa cggcagctac agcatgatgc
     2101 aggagcagct gggctacccg cagcacccgg gcctcaacgc tcacggcgcg gcacagatgc
     2161 aaccgatgca ccgctacgac gtcagcgccc tgcagtacaa ctccatgacc agctcgcaga
     2221 cctacatgaa cggctcgccc acctacagca tgtcctactc gcagcagggc acccccggta
     2281 tggcgctggg ctccatgggc tctgtggtca agtccgaggc cagctccagc ccccccgtgg
     2341 ttacctcttc ctcccactcc agggcgccct gccaggccgg ggacctccgg gacatgatca
     2401 gcatgtacct ccccggcgcc gaggtgccgg agcccgctgc gcccagtaga ctgcacatgg
     2461 cccagcacta ccagagcggc ccggtgcccg gcacggccat taacggcaca ctgcccctgt
     2521 cgcacatgtg aaacccagct ttcttgtaca aagtggtgat ggccgctcta gctagtcgat
     2581 aagctaattc cgcccccccc cccccccccc cctcaccctc ccccccccct aacgttactg
     2641 gccgaagccg cttggaataa ggccggtgag cgtttgtcta tatgttattt tccaccatat
     2701 tgccgtcttt tggcaatgtg agggcccgaa acctggccct gtcttcttga cgacgattcc
     2761 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     2821 agttcctctg gaagcttctt gaagacaaca acgtctgtag cgaccctttg caggcagcgg
     2881 aaccccccac ctggcgacat ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     2941 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     3001 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     3061 aaccacgggg acgtggtttt ggtttgaaaa acacgatgat aatatggcca caaccatgga
     3121 acagggggat cagcttacca tggccaagat ccctcctaag aagaagcgca aagtcgagga
     3181 tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3241 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3301 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga
     3361 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
     3421 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat
     3481 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
     3541 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa
     3601 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt
     3661 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat
     3721 ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg
     3781 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt
     3841 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg
     3901 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt
     3961 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga
     4021 tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa
     4081 tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc
     4141 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa
     4201 gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat
     4261 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt
     4321 gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct
     4381 gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac
     4441 cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga
     4501 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa
     4561 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga
     4621 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga
     4681 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc
     4741 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg
     4801 cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt
     4861 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc
     4921 ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt
     4981 ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt
     5041 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag
     5101 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga
     5161 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca
     5221 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc
     5281 atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag
     5341 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt
     5401 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca
     5461 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc
     5521 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt
     5581 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc
     5641 agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa
     5701 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac
     5761 cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca
     5821 gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc
     5881 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac
     5941 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg
     6001 cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact
     6061 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga
     6121 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt
     6181 ccagctgagc gccggtcgct accattacca gttggtctgg tgtcagggga tcccccgggc
     6241 tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc
     6301 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6361 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6421 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6481 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6541 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6601 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6661 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6721 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6781 caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc
     6841 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6901 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6961 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7021 cgccttctat cgccttcttg acgagttctt ctgaggggat caattctcta gggctcgctg
     7081 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7141 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7201 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7261 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7321 tgaggcggaa agaaccagct ggggctcgat cctctagagt cgagggctgc aggaattcga
     7381 tatcaagctc tagccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc
     7441 atcgtctaaa gaactaccca ttttattata tattagtcac gatatctata acaagaaaat
     7501 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     7561 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     7621 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     7681 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     7741 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taaaaaagat
     7801 ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg acaaaaacca
     7861 cattgtggcc aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga
     7921 ggtacccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc
     7981 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8041 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8101 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8161 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8221 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8281 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8341 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8821 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
