LOCUS       sequence-97560-e         298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 298)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-FLEX-EGFP
FEATURES             Location/Qualifiers
     source          1..298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(82..115)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(172..193)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta
       61 ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc
      121 gccctattac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      181 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
      241 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctgg
//
