LOCUS       sequence-97561-e         440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses splitTVA950.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 440)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-FLEX-splitTVA950
FEATURES             Location/Qualifiers
     source          1..440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    18..51
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    102..135
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ccgccaccat ggttaatata acttcgtata atgtatgcta tacgaagtta tgcagaatgg
       61 tagctggatt gtagctgcta ttagcaatat gaaacctctt aataacttcg tataaagtat
      121 cctatacgaa gttatggcgc gcctcaggag aacaagtctg cctggctctt gtcaggcacc
      181 agcagctcct tggatgcgct ttcaagactg aagatgtcag acctgctttt tgctttggac
      241 ttcccccatg cagcgatacc acccacagct accaggcagc acaggagcac tgcagtgatc
      301 agcatccaca tgcggccgtg attcctggct ggcagagcac gtccaggagc aggggcagtg
      361 ggagcctctg tgccgttgtc cgtgggcacc gcggggatcg cgctggtccc gcagccccac
      421 tcgtcccgcc cgtcgtcgca
//
