LOCUS       sequence-97573-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK17 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK17_amiE
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             330..352
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
       61 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
      121 ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggccacagc
      181 taacaccacg tcgtccctat ctgctgccct aggtctatga gtggttgctg gataacttcc
      241 cgggcatgca taaggctcgt aatatatatt cagggagacc acaacggttt ccctctacaa
      301 ataattttgt ttaactttct agaaataatt ttgtttaact ttaagaagga gatatacata
      361 tgagacatgg cgatattagc tcgtcaaatg ataccgtagg cgtagccgtg gtgaattaca
      421 agatgccgcg tttacatact gctgctgaag tcctggataa tgcccgcaaa attgcggaaa
      481 tgatcgttgg tatgaagcaa ggtctgccgg gcatggatct ggttgtgttt cctgaatatt
      541 ctttacaggg tattatgtac gaccctgctg aaatgatgga aacagccgtg gcgattccag
      601 gcgaagaaac ggaaatcttt agccgtgctt gtagaaaagc aaatgtttgg ggtgtgttct
      661 ccctgaccgg cgaacgtcat gaagaacacc cta
//
