LOCUS       sequence-97577-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK16 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK16_amiE
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgaggcctt catacggcag
       61 acgacctact gggcattggg cgacacctgt tgtggagcga gtcaggcgtt ccacgttttc
      121 tcttgccttt tcagcatcag taacccaggt acggtagaat tcaaatgggc attctgccag
      181 accacggtcg ccatcgcccg aagcctgcaa accggagtaa cctctgtgca agattttaaa
      241 caaatggttc tgactttggt cattcgcacg ggcatcgcgg atttgtgaca gagacaactg
      301 cgcgtattga atgcccattt cttcttcgcc gcattcaccc agagtacggc cgtcaaaacc
      361 gataatggca ctatgaccga agtagctata aacgccatca aaacctgcag cgtttgctac
      421 agcgacataa cagttattcg cccaggccat tgccttagcc atcataactt gttggtcttt
      481 agcagggtac atatagcctt ggcagcgaac gatcaattcc gcacccttca tggcacaatc
      541 tctccaaatt tccggataat taccgtcatc gcagataatt aatgagatct tcatgccttt
      601 cggaccttca gagacgtatg tctggccacc tggataccag ccttcaatcg gacaccatgg
      661 gatgatcttt ctgtactttt gtacgatttc accgttgtta tcgatcaaga ccagagtgtt
      721 gtatggtg
//
