LOCUS       sequence-97579-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK14 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK14_amiE
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgaggcct tcatacggca
       61 gacgacctac tgggcattgg gcgacacctg ttgtggagcg agtcaggcgt tccacgtttt
      121 ctcttgcctt ttcagcatca gtaacccagg tacggtagaa ttcaaatggg cattctgcca
      181 gaccacggtc gccatcgccc gaagcctgca aaccggagta acctctgtgc aagattttaa
      241 acaaatggtt ctgactttgg tcattcgcac gggcatcgcg gatttgtgac agagacaact
      301 gcgcgtattg aatgcccatt tcttcttcgc cgcattcacc cagagtacgg ccgtcaaaac
      361 cgataatggc actatgaccg aagtagctat aaacgccatc aaaacctgca gcgtttgcta
      421 cagcgacata acagttattc gcccaggcca ttgccttagc catcataact tgttggtctt
      481 tagcagggta catatagcct tggcagcgaa cgatcaattc cgcacccttc atggcacaat
      541 ctctccaaat ttccggataa ttaccgtcat cgcagataat taatgagatc ttcatgcctt
      601 tcggaccttc agagacgtat gtctggccac ctggatacca gccttcaatc ggacaccatg
      661 ggatgatctt tctgtacttt tgtacgattt caccgttgtt atcgatcaag accagagtgt
      721 tgtatggtgc ctttcta
//
