LOCUS       sequence-97581-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK9 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK9_amiE
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             334..356
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
       61 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
      121 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcca
      181 cagctaacac cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt gctggataac
      241 tttaccggca tgcataaggc tcgtaatata tattcaggga gaccacaacg gtttccctct
      301 acaaataatt ttgtttaact ttctagaaat aattttgttt aactttaaga aggagatata
      361 catatgagac atggcgatat tagctcgtca aatgataccg taggcgtagc cgtggtgaat
      421 tacaagatgc cgcgtttaca tactgctgct gaagtcctgg ataatgcccg caaaattgcg
      481 gaaatgatcg ttggtatgaa gcaaggtctg ccgggcatgg atctggttgt gtttcctgaa
      541 tattctttac agggtattat gtacgaccct gctgaaatga tggaaacagc cgtggcgatt
      601 ccaggcgaag aaacggaaat ctttagccgt gcttgtagaa aagcaaatgt ttggggtgtg
      661 ttctccctga ccggcgaacg tcatgaagaa caccctag
//
