LOCUS       sequence-97582-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK9 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK9_amiE
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag gccttcatac
       61 ggcagacgac ctactgggca ttgggcgaca cctgttgtgg agcgagtcag gcgttccacg
      121 ttttctcttg ccttttcagc atcagtaacc caggtacggt agaattcaaa tgggcattct
      181 gccagaccac ggtcgccatc gcccgaagcc tgcaaaccgg agtaacctct gtgcaagatt
      241 ttaaacaaat ggttctgact ttggtcattc gcacgggcat cgcggatttg tgacagagac
      301 aactgcgcgt attgaatgcc catttcttct tcgccgcatt cacccagagt acggccgtca
      361 aaaccgataa tggcactatg accgaagtag ctataaacgc catcaaaacc tgcagcgttt
      421 gctacagcga cataacagtt attcgcccag gccattgcct tagccatcat aacttgttgg
      481 tctttagcag ggtacatata gccttggcag cgaacgatca attccgcacc cttcatggca
      541 caatctctcc aaatttccgg ataattaccg tcatcgcaga taattaatga gatcttcatg
      601 cctttcggac cttcagagac gtatgtctgg ccacctggat accagccttc aatcggacac
      661 catgggatga tctttctgta cttttgtacg atttcaccgt tgttatcgat caagaccaga
      721 gtgttgtatg gtgcctttct a
//
