LOCUS       sequence-97583-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK6_amiE
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             327..349
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
       61 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
      121 cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg ccacagctaa
      181 caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat aacttgtcgg
      241 gcatgcataa ggctcgtaat atatattcag ggagaccaca acggtttccc tctacaaata
      301 attttgttta actttctaga aataattttg tttaacttta agaaggagat atacatatga
      361 gacatggcga tattagctcg tcaaatgata ccgtaggcgt agccgtggtg aattacaaga
      421 tgccgcgttt acatactgct gctgaagtcc tggataatgc ccgcaaaatt gcggaaatga
      481 tcgttggtat gaagcaaggt ctgccgggca tggatctggt tgtgtttcct gaatattctt
      541 tacagggtat tatgtacgac cctgctgaaa tgatggaaac agccgtggcg attccaggcg
      601 aagaaacgga aatctttagc cgtgcttgta gaaaagcaaa tgtttggggt gtgttctccc
      661 tgaccggcga acgtcatgaa gaacacccta gaaaggcacc atacaacact ctggtcttga
      721 tcgataacaa cggtg
//
