LOCUS       sequence-97585-e         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK3_amiE
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             271..293
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
       61 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggccacag
      121 ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct ggataacttt
      181 acgggcatgc ataaggctcg tagaatatat tcagggagac cacaacggtt tccctctaca
      241 aataattttg tttaactttc tagaaataat tttgtttaac tttaagaagg agatatacat
      301 atgagacatg gcgatattag ctcgtcaaat gataccgtag gcgtagccgt ggtgaattac
      361 aagatgccgc gtttacatac tgctgctgaa gtcctggata atgcccgcaa aattgcggaa
      421 atgatcgttg gtatgaagca aggtctgccg ggcatggatc tggttgtgtt tcctgaatat
      481 tctttacagg gtattatgta cgaccctgct gaaatgatgg aaacagccgt ggcgattcca
      541 ggcgaagaaa cggaaatctt tagccgtgct tgtagaaaag caaatgtttg gggtgtgttc
      601 tccctgaccg gcgaacgtca tgaagaacac cctag
//
