LOCUS       sequence-97586-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK3_amiE
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag gccttcatac ggcagacgac
       61 ctactgggca ttgggcgaca cctgttgtgg agcgagtcag gcgttccacg ttttctcttg
      121 ccttttcagc atcagtaacc caggtacggt agaattcaaa tgggcattct gccagaccac
      181 ggtcgccatc gcccgaagcc tgcaaaccgg agtaacctct gtgcaagatt ttaaacaaat
      241 ggttctgact ttggtcattc gcacgggcat cgcggatttg tgacagagac aactgcgcgt
      301 attgaatgcc catttcttct tcgccgcatt cacccagagt acggccgtca aaaccgataa
      361 tggcactatg accgaagtag ctataaacgc catcaaaacc tgcagcgttt gctacagcga
      421 cataacagtt attcgcccag gccattgcct tagccatcat aacttgttgg tctttagcag
      481 ggtacatata gccttggcag cgaacgatca attccgcacc cttcatggca caatctctcc
      541 aaatttccgg ataattaccg tcatcgcaga taattaatga gatcttcatg cctttcggac
      601 cttcagagac gtatgtctgg ccacctggat accagccttc aatcggacac catgggatga
      661 tctttctgta cttttgtacg a
//
