LOCUS       sequence-9759-ex       16680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Myc and Klf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16680)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 16680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S EM2K
FEATURES             Location/Qualifiers
     source          1..16680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..1175
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          244..1166
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    2385..2958
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2408..2960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    3977..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4641..5803
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4872..5794
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             5935..7254
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    7321..7894
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7344..7896
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    9381..9515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      10246..12035
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(12337..14262)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(14701..14719)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        14787..14891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14892..14960,14961..15752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15110..15129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15923..16511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16412..16431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
       61 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      301 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
      361 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      421 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      481 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      541 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt gggcccgcgg
      601 ccggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      661 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      721 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      781 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
      841 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
      901 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
      961 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1021 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1081 cgttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1141 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg tggtcgcggc caattctgca
     1201 gtcgagctca agcttcgaac tagtgattat ggcgggacac ctggcttcgg atttcgcctt
     1261 ctcgccccct ccaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga
     1321 tcctcggacc tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt
     1381 tgggccaggc tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg
     1441 ggggatggcg tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga
     1501 gacctctcag cctgagggtg aagcangagt cggggtggag agcaactccg atggggcctc
     1561 cccggagccc tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca
     1621 aaacccggag gagtcccagg acatcaaagc tctgcagaaa gaactcgagc aatttgccaa
     1681 gctcctgaag cagaagagga tcaccctggg atatacacag gccgatgtgg ggctcaccct
     1741 gggggttcta tttgggaagg tattcagcca aacgaccatc tgccgctttg aggctctgca
     1801 gcttagcttc aagaacatgt gtaagctgcg gcccttgctg cagaagtggg tggaggaagc
     1861 tgacaacaat gaaaatcttc aggagatatg caaagcagaa accctcgtgc aggcccgaaa
     1921 gagaaagcga accagtatcg agaaccgagt gagaggcaac ctggagaatt tgttcctgca
     1981 gtgcccgaaa cccacactgc agcagatcag ccacatcgcc cagcagcttg ggctcgagaa
     2041 ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag ggcaagcgat caagcagcga
     2101 ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc
     2161 ctttcctctg gccccagggc cccattttgg taccccaggc tatgggagcc ctcacttcac
     2221 tgcactgtac tcctcggtcc ctttccctga gggggaagcc tttccccctg tctccgtcac
     2281 cactctgggc tctcccatgc attcaaactg aggtgcctgc cctctagttt acgcgtgggg
     2341 gcggccggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
     2401 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2461 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2521 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2581 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2641 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2701 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2761 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2821 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
     2881 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     2941 tgaaaaacac gatgataata tggccacacc gcgaattaat tcgattccgc atgtacaaca
     3001 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
     3061 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
     3121 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
     3181 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
     3241 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
     3301 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
     3361 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
     3421 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
     3481 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
     3541 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
     3601 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3661 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
     3721 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
     3781 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
     3841 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
     3901 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagggccg gaaatcacta
     3961 gctagttcga aggatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4201 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4321 gcccgtcagg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgaccaat
     4381 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
     4441 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
     4501 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     4561 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
     4621 attattgact agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     4681 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     4741 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     4801 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     4861 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     4921 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
     4981 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
     5041 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     5101 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     5161 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
     5221 gcccgcggcc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5281 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5341 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5401 accagttgcg tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg
     5461 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5521 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5581 cgattagttc tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5641 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5701 gtaattctcg ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5761 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacacgtg gtcgcggcca
     5821 attctgcagt cgagctcaag cttcgaacta gggccgcgac tctagaagct gggtaccagc
     5881 tgctagcgct accggactca gatctcgagc tcaagcttcg aattcgcgat cgccatgccc
     5941 ctcaacgtta gcttcaccaa caggaactat gacctcgact acgactcggt gcagccgtat
     6001 ttctactgcg acgaggagga gaacttctac cagcagcagc agcagagcga gctgcagccc
     6061 ccggcgccca gcgaggatat ctggaagaaa ttcgagctgc tgcccacccc gcccctgtcc
     6121 cctagccgcc gctccgggct ctgctcgccc tcctacgttg cggtcacacc cttctccctt
     6181 cggggagaca acgacggcgg tggcgggagc ttctccacgg ccgaccagct ggagatggtg
     6241 accgagctgc tgggaggaga catggtgaac cagagtttca tctgcgaccc ggacgacgag
     6301 accttcatca aaaacatcat catccaggac tgtatgtgga gcggcttctc ggccgccgcc
     6361 aagctcgtct cagagaagct ggcctcctac caggctgcgc gcaaagacag cggcagcccg
     6421 aaccccgccc gcggccacag cgtctgctcc acctccagct tgtacctgca ggatctgagc
     6481 gccgccgcct cagagtgcat cgacccctcg gtggtcttcc cctaccctct caacgacagc
     6541 agctcgccca agtcctgcgc ctcgcaagac tccagcgcct tctctccgtc ctcggattct
     6601 ctgctctcct cgacggagtc ctccccgcag ggcagccccg agcccctggt gctccatgag
     6661 gagacaccgc ccaccaccag cagcgactct gaggaggaac aagaagatga ggaagaaatc
     6721 gatgttgttt ctgtggaaaa gaggcaggct cctggcaaaa ggtcagagtc tggatcacct
     6781 tctgctggag gccacagcaa acctcctcac agcccactgg tcctcaagag gtgccacgtc
     6841 tccacacatc agcacaacta cgcagcgcct ccctccactc ggaaggacta tcctgctgcc
     6901 aagagggtca agttggacag tgtcagagtc ctgagacaga tcagcaacaa ccgaaaatgc
     6961 accagcccca ggtcctcgga caccgaggag aatgtcaaga ggcgaacaca caacgtcttg
     7021 gagcgccaga ggaggaacga gctaaaacgg agcttttttg ccctgcgtga ccagatcccg
     7081 gagttggaaa acaatgaaaa ggcccccaag gtagttatcc ttaaaaaagc cacagcatac
     7141 atcctgtccg tccaagcaga ggagcaaaag ctcatttctg aagaggactt gttgcggaaa
     7201 cgacgagaac agttgaaaca caaacttgaa cagctacgga actcttgtgc gtaagtttaa
     7261 acatcgatgg cctcgagaaa gcggccgctt tctcgaggcc atcgatgttt aaacgatccg
     7321 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     7381 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     7441 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     7501 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     7561 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     7621 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     7681 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7741 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta
     7801 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     7861 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaccgcga tcgcgaattc
     7921 gcgatcgcca tggctgtcag cgacgcgctg ctcccatctt tctccacgtt cgcgtctggc
     7981 ccggcgggaa gggagaagac actgcgtcaa gcaggtgccc cgaataaccg ctggcgggag
     8041 gagctctccc acatgaagcg acttccccca gtgcttcccg gccgccccta tgacctggcg
     8101 gcggcgaccg tggccacaga cctggagagc ggcggagccg gtgcggcttg cggcggtagc
     8161 aacctggcgc ccctacctcg gagagagacc gaggagttca acgatctcct ggacctggac
     8221 tttattctct ccaattcgct gacccatcct ccggagtcag tggccgccac cgtgtcctcg
     8281 tcagcgtcag cctcctcttc gtcgtcgccg tcgagcagcg gccctgccag cgcgccctcc
     8341 acctgcagct tcacctatcc gatccgggcc gggaacgacc cgggcgtggc gccgggcggc
     8401 acgggcggag gcctcctcta tggcagggag tccgctcccc ctccgacggc tcccttcaac
     8461 ctggcggaca tcaacgacgt gagcccctcg ggcggcttca tggccgagct cctgcggcca
     8521 gaattggacc cggtgtacat tccgccgcag cagccgcagc cgccaggtgg cgggctgatg
     8581 ggcaagttcg tgctgaaggc gtcgctgagc gcccctggca gcgagtacgg cagcccgtcg
     8641 gtcatcagcg tcagcaaagg cagccctgac ggcagccacc cggtggtggt ggcgccctac
     8701 aacggcgggc cgccgcgcac gtgccccaag atcaagcagg aggcggtctc ttcgtgcacc
     8761 cacttgggcg ctggaccccc tctcagcaat ggccaccggc cggctgcaca cgacttcccc
     8821 ctggggcggc agctccccag caggactacc ccgaccctgg gtcttgagga agtgctgagc
     8881 agcagggact gtcaccctgc cctgccgctt cctcccggct tccatcccca cccggggccc
     8941 aattacccat ccttcctgcc cgatcagatg cagccgcaag tcccgccgct ccattaccaa
     9001 gagctcatgc cacccggttc ctgcatgcca gaggagccca agccaaagag gggaagacga
     9061 tcgtggcccc ggaaaaggac cgccacccac acttgtgatt acgcgggctg cggcaaaacc
     9121 tacacaaaga gttcccatct caaggcacac ctgcgaaccc acacaggtga gaaaccttac
     9181 cactgtgact gggacggctg tggatggaaa ttcgcccgct cagatgaact gaccaggcac
     9241 taccgtaaac acacggggca ccgcccgttc cagtgccaaa aatgcgaccg agcattttcc
     9301 aggtcggacc acctcgcctt acacatgaag aggcattttt aaatcccaga cagtggatat
     9361 gacccgtttc tagttcgaag gatccagaca tgataagata cattgatgag tttggacaaa
     9421 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     9481 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     9541 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     9601 gtggtatggc tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     9661 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     9721 acaagcccgt caggcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgat
     9781 cgactctagc tagaggatcg atgccccgcc ccggacgaac taaacctgac tacgacatct
     9841 ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca
     9901 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact
     9961 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct
    10021 ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg
    10081 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg
    10141 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa
    10201 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg
    10261 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc
    10321 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat
    10381 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag
    10441 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc
    10501 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg
    10561 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat
    10621 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca
    10681 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata
    10741 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg
    10801 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca
    10861 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag
    10921 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg
    10981 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt
    11041 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata
    11101 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc
    11161 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata
    11221 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
    11281 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
    11341 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
    11401 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
    11461 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
    11521 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
    11581 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
    11641 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
    11701 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
    11761 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
    11821 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
    11881 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag
    11941 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa
    12001 gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa
    12061 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
    12121 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
    12181 gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca
    12241 ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct
    12301 tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc
    12361 atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc
    12421 caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac
    12481 cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa
    12541 aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct
    12601 tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg
    12661 tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt
    12721 cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc
    12781 tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt
    12841 ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc
    12901 accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc
    12961 tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa
    13021 ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg
    13081 tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc
    13141 acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc
    13201 ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc
    13261 actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc
    13321 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
    13381 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
    13441 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
    13501 tcctgcccct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc
    13561 ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
    13621 tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
    13681 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
    13741 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
    13801 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
    13861 tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
    13921 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc
    13981 tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt
    14041 ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc
    14101 tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc
    14161 tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc
    14221 taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga
    14281 tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc
    14341 ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg
    14401 aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa
    14461 actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct
    14521 cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt
    14581 caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg
    14641 aggatcagct tatcgatgat aagctgtcaa acatgagaat taattcttga agacgaaagg
    14701 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    14761 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    14821 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    14881 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    14941 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    15001 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    15061 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    15121 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    15181 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    15241 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    15301 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    15361 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    15421 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    15481 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    15541 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    15601 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    15661 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    15721 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    15781 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    15841 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    15901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    15961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    16021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
    16081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    16141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    16201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    16261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    16321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    16381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    16441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    16501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    16561 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
    16621 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
//
