LOCUS       sequence-97596-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses eGFP with proK16 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK16_eGFP
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(87..107)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     702..730
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtttg tatagttcat
       61 ccatgccatg tgtaatccca gcagctgtta caaactcaag aaggaccatg tggtctctct
      121 tttcgttggg atctttcgaa agggcagatt gtgtggacag gtaatggttg tctggtaaaa
      181 ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcttc
      241 catcttcaat gttgtgtcta attttgaagt taactttgat tccattcttt tgtttgtctg
      301 ccatgatgta tacattgtgt gagttatagt tgtattccaa tttgtgtcca agaatgtttc
      361 catcttcttt aaaatcaata ccttttaact cgattctatt aacaagggta tcaccttcaa
      421 acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt gaaaaatata gttctttcct
      481 gtacataacc ttcgggcatg gcactcttga aaaagtcatg ctgtttcata tgatctgggt
      541 atctcgcaaa gcattgaaca ccataaccga aagtagtgac aagtgttggc catggaacag
      601 gtagttttcc agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt
      661 caccctctcc actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa
      721 ttgggacaac tccagtg
//
