LOCUS       sequence-97597-e         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses eGFP with proK14 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK14_eGFP
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             323..345
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(383..411)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
       61 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
      121 aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggccac agctaacacc
      181 acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact gtacgggcat
      241 gcataaggct cgtaatatat attcagggag accacaacgg tttccctcta caaataattt
      301 tgtttaactt tctagaaata attttgttta actttaagaa ggagatatac atatgcgtaa
      361 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      421 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      481 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      541 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
      601 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tc
//
